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Musashi-1 Antibody (OTI1G8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03434

Novus Biologicals, part of Bio-Techne
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NBP2-03434

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1G8

Concentration

1 mg/ml

Product Summary for Musashi-1 Antibody (OTI1G8)

Immunogen

Full length human recombinant protein of human MSI1 (NP_002433) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Neuronal Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Musashi-1 Antibody (OTI1G8)

Western Blot: Musashi 1 Antibody (1G8) [NBP2-03434] Analysis of extracts (35ug) from 9 different cell lines by using anti-Musashi 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody (OTI1G8) [NBP2-03434]

Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody (OTI1G8) [NBP2-03434]

Immunocytochemistry/Immunofluorescence: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Musashi 1.
Immunohistochemistry-Paraffin: Musashi-1 Antibody (OTI1G8) [NBP2-03434]

Immunohistochemistry-Paraffin: Musashi-1 Antibody (OTI1G8) [NBP2-03434]

Immunohistochemistry-Paraffin: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of paraffin-embedded Human pancreas tissue using anti-Musashi 1 mouse monoclonal antibody.

Applications for Musashi-1 Antibody (OTI1G8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Musashi-1

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq].

Alternate Names

MSI1, Musashi1

Entrez Gene IDs

4440 (Human)

Gene Symbol

MSI1

Additional Musashi-1 Products

Product Documents for Musashi-1 Antibody (OTI1G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Musashi-1 Antibody (OTI1G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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