N-Cadherin Antibody (8C11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54523
![Western Blot: N-Cadherin Antibody (8C11)Azide and BSA Free [NBP2-54523]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-8C11-Azide-and-BSA-Free-Western-Blot-NBP2-54523-img0002.jpg "Western Blot: N-Cadherin Antibody (8C11)Azide and BSA Free [NBP2-54523]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8C11
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human N-Cadherin extracellular domain (Uniprot: P19022 )
Reactivity Notes
Human and Mouse. Others not known.
Localization
Cell surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53321).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for N-Cadherin Antibody (8C11) - Azide and BSA Free
Western Blot: N-Cadherin Antibody (8C11)Azide and BSA Free [NBP2-54523]
Western Blot: N-Cadherin Antibody (8C11) - Azide and BSA Free [NBP2-54523] - Western blot analysis of Heart tissue lysate using N-Cadherin antibody (8C11).![Immunohistochemistry-Paraffin: N-Cadherin Antibody (8C11) - Azide and BSA Free [NBP2-54523]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-8C11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54523-img0001.jpg "Immunohistochemistry-Paraffin: N-Cadherin Antibody (8C11) - Azide and BSA Free [NBP2-54523]")
Immunohistochemistry-Paraffin: N-Cadherin Antibody (8C11) - Azide and BSA Free [NBP2-54523]
Immunohistochemistry-Paraffin: N-Cadherin Antibody (8C11) - Azide and BSA Free [NBP2-54523] - Mouse heart stained with N-Cadherein antibody (8C11).Applications for N-Cadherin Antibody (8C11) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: N-Cadherin
N-cadherin is expressed on multiple cell types but is most highly expressed by mesenchymal cells and neural tissue (2). Functionally, N-cadherin has a number of roles including maintaining structural integrity and adhesion, cell signaling, and formation of neuronal synapses and the vascular wall (2). The cytoplasmic tail interacts with beta-catenin which then binds with alpha-catenin, forming the cadherin-catenin adhesion complex, an important component of adhesions junctions (1-3). Given its role in adhesion, N-cadherin serves as an indicator of epithelial-to-mesenchymal transition (EMT) (1-4). The loss of E-cadherin during EMT corresponds with an increase in N-cadherin expression (1-4). This "cadherin-switch" is associated with increased migratory and invasive behavior observed in tumor progress (1-4). Proteases including activity of a disintegrin and metalloprotease 10 (ADAM10), matrix metalloproteinases (MMPs), caspase 3, presenilin, and calpain can cleave N-cadherin as a mechanism for regulating Wnt/beta-catenin signaling and inducing oncogenic signals (3,4). In addition to its expression in solid tumors, N-cadherin has been indicated in hematological disorders such as leukemia and multiple myeloma (1). N-cadherin antagonists are currently being studied as potential therapeutics for a variety of cancer studies (1-2).
References
1. Mrozik, K. M., Blaschuk, O. W., Cheong, C. M., Zannettino, A., & Vandyke, K. (2018). N-cadherin in cancer metastasis, its emerging role in haematological malignancies and potential as a therapeutic target in cancer. BMC Cancer. https://doi.org/10.1186/s12885-018-4845-0
2. Loh, C. Y., Chai, J. Y., Tang, T. F., Wong, W. F., Sethi, G., Shanmugam, M. K., Chong, P. P., & Looi, C. Y. (2019). The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges. Cells. https://doi.org/10.3390/cells8101118
3. Derycke, L. D., & Bracke, M. E. (2004). N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling. The International Journal of Developmental Biology. https://doi.org/10.1387/ijdb.041793ld
4. Yu, W., Yang, L., Li, T., & Zhang, Y. (2019). Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target. Frontiers in Oncology. https://doi.org/10.3389/fonc.2019.00989
5. Unitprot (P1903)
Long Name
Neural Cadherin
Alternate Names
Cadherin-2, CD325, CDH2, NCadherin
Gene Symbol
CDH2
Additional N-Cadherin Products
Product Documents for N-Cadherin Antibody (8C11) - Azide and BSA Free
Product Specific Notices for N-Cadherin Antibody (8C11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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