Nbs1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06609
![Immunohistochemistry-Paraffin: Nbs1 Antibody - BSA Free [NBP1-06609]](https://resources.bio-techne.com/images/products/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06609-img0003.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody - BSA Free [NBP1-06609]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Nbs1 Antibody was made to a synthetic peptide made to an internal portion of the mouse NBS1 protein (within residues 350-400). [Swiss-Prot# Q9R207]
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 21349997 and 30176843). Mouse reactivity reported in scientific literature.
Localization
Nucleus. Telomere. Note= Localizes to discrete nuclear foci after treatment with genotoxic agents.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nbs1 Antibody - BSA Free
Immunohistochemistry-Paraffin: Nbs1 Antibody - BSA Free [NBP1-06609]
Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].![Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609]](https://resources.bio-techne.com/images/products/Nbs1-Antibody-Immunohistochemistry-NBP1-06609-img0005.jpg "Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609]")
Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609]
Immunohistochemistry: Nbs1 Antibody [NBP1-06609] - Immunohistochemical staining of NBS1 proteins. Representative examples of typical nuclear staining of NBS1(c) scored as high expression in tumor cells. Correspondingly, examples scored as low expression (f) is shown (40x magnification). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4776-9) licensed under a CC-BY license.![Western Blot: Nbs1 AntibodyBSA Free [NBP1-06609]](https://resources.bio-techne.com/images/products/NBS1-Antibody-Western-Blot-NBP1-06609-img0004.jpg "Western Blot: Nbs1 AntibodyBSA Free [NBP1-06609]")
Western Blot: Nbs1 AntibodyBSA Free [NBP1-06609]
Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.Applications for Nbs1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
2 ug/mL
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 21349997; 30176843)
Western Blot
2 ug/mL. Use reported in scientific literature
Application Notes
In Western blot a band is seen at ~97 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 30% Glycerol
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nbs1
In DNA double strand break repair, the FHA/BRCT domains bind DNA damage sensor proteins including gamma H2AX (phospho Ser139), phosphorylated MDC1 and CtIP (CtBP-interacting protein). NBS1 then recruits the other members of the MRN complex, Mre11 and Rad50, to the proximity of DNA DSBs. The MRN complex has also been shown to interact with ATM or ATR kinases in the presence of DSBs or replication fork stalling, respectively. Phosphorylation of NBS1 at Ser 278 and Ser343 in response to ionizing radiation (IR) is dependent on ATM and is important for activation of the S phase checkpoint. The activation of ATM in response to DNA damage is also facilitated by MRN and may lead to induction of apoptosis (2, 3).
References
1. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 26;18(1):169. PMID: 31767017
2. Zhang Y, Zhou J, Lim CU. (2006) The role of NBS1 in DNA double strand break repair, telomere stability, and cell cycle checkpoint control. Cell Res. 16(1):45-54. PMID: 16467875
3. Komatsu K. NBS1 and multiple regulations of DNA damage response. (2016) J Radiat Res. 57 Suppl 1:i11-i17. PMID: 27068998
Long Name
Nijmegen Breakage Syndrome 1
Alternate Names
NBN, Nibrin, p95
Gene Symbol
NBN
Additional Nbs1 Products
Product Documents for Nbs1 Antibody - BSA Free
Product Specific Notices for Nbs1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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