NEK6 Antibody (OTI5D7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47865

Novus Biologicals, part of Bio-Techne
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NBP1-47865
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-72938AF350
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Alexa Fluor 405
NBP2-72938AF405
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Alexa Fluor 488
NBP2-72938AF488
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Alexa Fluor 532
NBP2-72938AF532
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Alexa Fluor 594
NBP2-72938AF594
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Alexa Fluor 647
NBP2-72938AF647
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Alexa Fluor 700
NBP2-72938AF700
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Alexa Fluor 750
NBP2-72938AF750
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Allophycocyanin
NBP2-72938APC
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Allophycocyanin/Cy7
NBP2-72938APCCY7
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Biotin
NBP2-72938B
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CoraFluor 1
NBP2-72938CL1
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DyLight 350
NBP2-72938UV
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DyLight 405
NBP2-72938V
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DyLight 488
NBP2-72938G
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DyLight 550
NBP2-72938R
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DyLight 594
NBP2-72938DL594
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DyLight 650
NBP2-72938C
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DyLight 680
NBP2-72938FR
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DyLight 755
NBP2-72938IR
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FITC
NBP2-72938F
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HRP
NBP2-72938H
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Janelia Fluor 525
NBP2-72938JF525
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Janelia Fluor 549
NBP2-72938JF549
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Janelia Fluor 585
NBP2-72938JF585
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Janelia Fluor 635
NBP2-72938JF635
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Janelia Fluor 646
NBP2-72938JF646
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Janelia Fluor 669
NBP2-72938JF669
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mFluor Violet 450 SE
NBP2-72938MFV450
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mFluor Violet 500 SE
NBP2-72938MFV500
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mFluor Violet 610 SE
NBP2-72938MFV610
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PE
NBP2-72938PE
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PE/Atto594
NBP2-72938PEATT594
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PE/Cy5.5
NBP2-72938PECY55
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PE/Cy7
NBP2-72938PECY7
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PerCP
NBP2-72938PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5D7

Concentration

0.75 mg/ml

View all NEK6 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens NIMA (never in mitosis gene a)-related kinase 6 (NEK6), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK6 Antibody (OTI5D7)

Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865]

Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865]

Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
Immunocytochemistry/ Immunofluorescence: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunocytochemistry/ Immunofluorescence: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunocytochemistry/Immunofluorescence: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6 .
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NEK6 mouse monoclonal antibody.
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Applications for NEK6 Antibody (OTI5D7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.75 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK6

The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA.

Alternate Names

EC 2.7.11.1, Never in mitosis A-related kinase 6, NIMA (never in mitosis gene a)-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512, putative serine-threonine protein kinase, serine/threonine-protein kinase Nek6, SID6-1512

Entrez Gene IDs

10783 (Human)

Gene Symbol

NEK6

UniProt

Q9HC98 (Human)

Additional NEK6 Products

Product Documents for NEK6 Antibody (OTI5D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEK6 Antibody (OTI5D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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