NEK6 Antibody (OTI5D7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72938

Novus Biologicals, part of Bio-Techne
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NBP2-72938
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Conjugate
Catalog #
Unconjugated
NBP1-47865
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Alexa Fluor 350
NBP2-72938AF350
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Alexa Fluor 405
NBP2-72938AF405
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Alexa Fluor 488
NBP2-72938AF488
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Alexa Fluor 532
NBP2-72938AF532
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Alexa Fluor 594
NBP2-72938AF594
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Alexa Fluor 647
NBP2-72938AF647
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Alexa Fluor 700
NBP2-72938AF700
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Alexa Fluor 750
NBP2-72938AF750
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Allophycocyanin
NBP2-72938APC
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Allophycocyanin/Cy7
NBP2-72938APCCY7
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Biotin
NBP2-72938B
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CoraFluor 1
NBP2-72938CL1
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DyLight 350
NBP2-72938UV
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DyLight 405
NBP2-72938V
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DyLight 488
NBP2-72938G
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DyLight 550
NBP2-72938R
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DyLight 594
NBP2-72938DL594
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DyLight 650
NBP2-72938C
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DyLight 680
NBP2-72938FR
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DyLight 755
NBP2-72938IR
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FITC
NBP2-72938F
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HRP
NBP2-72938H
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Janelia Fluor 525
NBP2-72938JF525
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Janelia Fluor 549
NBP2-72938JF549
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Janelia Fluor 585
NBP2-72938JF585
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Janelia Fluor 635
NBP2-72938JF635
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Janelia Fluor 646
NBP2-72938JF646
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Janelia Fluor 669
NBP2-72938JF669
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mFluor Violet 450 SE
NBP2-72938MFV450
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mFluor Violet 500 SE
NBP2-72938MFV500
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mFluor Violet 610 SE
NBP2-72938MFV610
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PE
NBP2-72938PE
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PE/Atto594
NBP2-72938PEATT594
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PE/Cy5.5
NBP2-72938PECY55
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PE/Cy7
NBP2-72938PECY7
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PerCP
NBP2-72938PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5D7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NEK6 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK6 Antibody (OTI5D7) - Azide and BSA Free

Western Blot: NEK6 Antibody (OTI5D7)Azide and BSA Free [NBP2-72938]

Western Blot: NEK6 Antibody (OTI5D7)Azide and BSA Free [NBP2-72938]

Western Blot: NEK6 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72938] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
Immunohistochemistry: NEK6 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72938]

Immunohistochemistry: NEK6 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72938]

Immunohistochemistry: NEK6 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72938] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NEK6 mouse monoclonal antibody.
Flow Cytometry: NEK6 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72938]

Flow Cytometry: NEK6 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72938]

Flow Cytometry: NEK6 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72938] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6 .
View 7 more images

Applications for NEK6 Antibody (OTI5D7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK6

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

Alternate Names

EC 2.7.11.1, Never in mitosis A-related kinase 6, NIMA (never in mitosis gene a)-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512, putative serine-threonine protein kinase, serine/threonine-protein kinase Nek6, SID6-1512

Gene Symbol

NEK6

Additional NEK6 Products

Product Documents for NEK6 Antibody (OTI5D7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEK6 Antibody (OTI5D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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