Nestin Antibody (10C2) [DyLight 550]
Novus Biologicals, part of Bio-Techne | Catalog # NB300-266R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rabbit (Negative), Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
DyLight 550 (Excitation = 562 nm, Emission = 576 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 10C2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
Reactivity Notes
This antibody does not cross-react with rat or rabbit.
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Nestin Antibody (10C2) [DyLight 550]
Flow Cytometry: Nestin Antibody (10C2) [DyLight 550] [NB300-266R] - An intracellular stain was performed on SK-MEL-28 cells with Nestin [10C2] Antibody NB300-266R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Flow Cytometry: Nestin Antibody (10C2) [DyLight 550] [NB300-266R] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Applications for Nestin Antibody (10C2) [DyLight 550]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Nestin
Alternate Names
NES
Gene Symbol
NES
Additional Nestin Products
Product Documents for Nestin Antibody (10C2) [DyLight 550]
Product Specific Notices for Nestin Antibody (10C2) [DyLight 550]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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