Nestin Antibody (10C2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80869

Novus Biologicals, part of Bio-Techne
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NBP2-80869
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Catalog #
BSA Free
NB300-266
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rabbit (Negative), Rat (Negative)

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 10C2

Format

Azide and BSA Free

Concentration

2.4 mg/ml

View all Nestin Antibodies »

Product Summary for Nestin Antibody (10C2) - Azide and BSA Free

Immunogen

A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]

Reactivity Notes

It does not cross-react in rat or rabbit.

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Nestin Antibody (10C2) - Azide and BSA Free

Western Blot: Nestin Antibody (10C2)Azide and BSA Free [NBP2-80869]

Western Blot: Nestin Antibody (10C2)Azide and BSA Free [NBP2-80869]

Western Blot: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein. Image from the standard format of this antibody.
Immunocytochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Immunocytochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Immunocytochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection re
Immunohistochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Immunohistochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Immunohistochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - GFAP and Nestin immunoreactivity in retinal sections. Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at
View 5 more images

Applications for Nestin Antibody (10C2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Frozen

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100 - 1:200

Western Blot

1:1000 - 1:5000
Application Notes
This Nestin (10C2) antibody is useful for Immunohistochemistry on formaldehyde fixed cultured cells and formalin fixed paraffin-embedded tissue sections. In Western blot a doublet band is seen at ~220-240 kDa. The investigator should determine the optimal working dilution for a specific application. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

2.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Alternate Names

NES

Gene Symbol

NES

Additional Nestin Products

Product Documents for Nestin Antibody (10C2) - Azide and BSA Free

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Product Specific Notices for Nestin Antibody (10C2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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