NF-H Antibody (RT-97 + NR-4) [PerCP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47664PCP
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Chicken
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
PerCP (Excitation = 490 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # RT-97 + NR-4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NF-H Antibody (RT-97 + NR-4) [PerCP]
Immunogen
Triton-X 100 insoluble proteins of rat brain (RT-97); Neurofilaments from porcine spinal cord (NR-4) (Uniprot: P12036)
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Marker
Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG1 Kappa
Applications for NF-H Antibody (RT-97 + NR-4) [PerCP]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: NF-H
Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Heavy Chain
Alternate Names
NEFH, NFH
Gene Symbol
NEFH
Additional NF-H Products
Product Documents for NF-H Antibody (RT-97 + NR-4) [PerCP]
Product Specific Notices for NF-H Antibody (RT-97 + NR-4) [PerCP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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