NFkB1/NFkB p105 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77395

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all NFkB1 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an N-terminal region of the human NFkB p105/p50 protein (within residues 400-590). [Swiss-Prot P19838]

Localization

Nucleus and cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFkB1/NFkB p105 Antibody - BSA Free

Western Blot: NFkB1/NFkB p105 AntibodyBSA Free [NBP1-77395]

Western Blot: NFkB1/NFkB p105 AntibodyBSA Free [NBP1-77395]

Western Blot: NFkB1/NFkB p105 Antibody [NBP1-77395] - WB analysis of NFkB in HepG2 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: NFkB1/NFkB p105 Antibody - BSA Free [NBP1-77395]

Immunocytochemistry/ Immunofluorescence: NFkB1/NFkB p105 Antibody - BSA Free [NBP1-77395]

Immunocytochemistry/Immunofluorescence: NFkB1/NFkB p105 Antibody [NBP1-77395] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: NFkB1/NFkB p105 Antibody - BSA Free [NBP1-77395]

Immunohistochemistry: NFkB1/NFkB p105 Antibody - BSA Free [NBP1-77395]

Immunohistochemistry: NFkB1/NFkB p105 Antibody [NBP1-77395] - IHC analysis of NFKB in mouse kidney using DAB with hematoxylin counterstain.
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Applications for NFkB1/NFkB p105 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:250

Immunohistochemistry-Frozen

reported in scientific literature (PMID 26889045)

Immunohistochemistry-Paraffin

1:250

Simple Western

1:20

Western Blot

1:1000
Application Notes
In Western blot, a band is seen approx. 110 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB1

Nuclear factor kappa beta (NFKB) is a transcription factor that is highly influenced by stress, free-radicals, UV light and hypoxia. Unsurprisingly, NFKB is essential for many human cancers, as it regulates the expression of many downstream proteins that regulate or modify cellular growth and development. NFKB effects on angiogenic pathways and reactive mechanisms to stress and damage are well established throughout literature. Atherosclerosis is partly attributed to the mis-regulation of NFKB in hypoxia. Arthritis is related to inflammation proteins regulated through NFKB. It also has been shown to be important in synapses in brain, leading to memory and cognitive ability or impairment. In all, NFKB is one of the fastest responding transcription factors in humans, and is likely to mediate many responsive events in diseases and injury recovery.

Long Name

Nuclear Factor Kappa-B, Subunit 1

Alternate Names

NF-kB1

Entrez Gene IDs

4790 (Human); 18033 (Mouse)

Gene Symbol

NFKB1

UniProt

P19838, P25799 (Mouse)

Additional NFkB1 Products

Product Documents for NFkB1/NFkB p105 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFkB1/NFkB p105 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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