NFkB2/NFkB p100 [p Ser866] Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16894
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S866 of human NFkB2/NFkB p100 (NP_001070962.1). EDSAY
Modification
p Ser866
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NFkB2/NFkB p100 [p Ser866] Antibody - Azide and BSA Free
Western Blot: NFkB2/NFkB p100 [p Ser866] AntibodyAzide and BSA Free [NBP3-16894]
Western Blot: NFkB2/NFkB p100 [p Ser866] Antibody [NBP3-16894] - Western blot analysis of extracts of MDA-MB435 cells, using NFkB2/NFkB p100 antibody (NBP3-16894) at dilution. MDA-MB435 cells were treated by TNF-alpha. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.Immunocytochemistry/ Immunofluorescence: NFkB2/NFkB p100 [p Ser866] Antibody - Azide and BSA Free [NBP3-16894]
Immunocytochemistry/Immunofluorescence: NFkB2/NFkB p100 [p Ser866] Antibody [NBP3-16894] - Immunofluorescence analysis of methanol-fixed HeLa cells using NFkB2/NFkB p100 antibody (NBP3-16894).Immunohistochemistry-Paraffin: NFkB2/NFkB p100 [p Ser866] Antibody - Azide and BSA Free [NBP3-16894]
Immunohistochemistry-Paraffin: NFkB2/NFkB p100 [p Ser866] Antibody [NBP3-16894] - Immunohistochemistry of paraffin-embedded human breast carcinoma using NFkB2/NFkB p100 antibody (NBP3-16894).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for NFkB2/NFkB p100 [p Ser866] Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100
Immunohistochemistry
1:50 - 1:100
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NFkB2
Long Name
Nuclear Factor Kappa-B, Subunit 2
Alternate Names
LYT10, NF-kB2
Gene Symbol
NFKB2
Additional NFkB2 Products
Product Documents for NFkB2/NFkB p100 [p Ser866] Antibody - Azide and BSA Free
Product Specific Notices for NFkB2/NFkB p100 [p Ser866] Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov