NFX1 Antibody (1D12)
Novus Biologicals, part of Bio-Techne | Catalog # H00004799-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1D12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NFX1 (NP_002495, 981 a.a. ~ 1080 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREM
Localization
Nuclear
Specificity
NFX1 - nuclear transcription factor, X-box binding 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NFX1 Antibody (1D12)
![Western Blot: NFX1 Antibody (1D12) [H00004799-M01]](https://resources.bio-techne.com/images/products/NFX1-Antibody-1D12-Western-Blot-H00004799-M01-img0007.jpg "Western Blot: NFX1 Antibody (1D12) [H00004799-M01]")
Western Blot: NFX1 Antibody (1D12) [H00004799-M01]
Western Blot: NFX1 Antibody (1D12) [H00004799-M01] - NFX1 monoclonal antibody (M01), clone 1D12 Analysis of NFX1 expression in Hela S3 NE.![Immunocytochemistry/ Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01]](https://resources.bio-techne.com/images/products/NFX1-Antibody-1D12-Immunocytochemistry-Immunofluorescence-H00004799-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01]")
Immunocytochemistry/ Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01]
Immunocytochemistry/Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01] - Analysis of monoclonal antibody to NFX1 on HeLa cell. Antibody concentration 30 ug/ml.![ELISA: NFX1 Antibody (1D12) [H00004799-M01]](https://resources.bio-techne.com/images/products/NFX1-Antibody-1D12-ELISA-H00004799-M01-img0006.jpg "ELISA: NFX1 Antibody (1D12) [H00004799-M01]")
ELISA: NFX1 Antibody (1D12) [H00004799-M01]
ELISA: NFX1 Antibody (1D12) [H00004799-M01] - Detection limit for recombinant GST tagged NFX1 is approximately 0.1ng/ml as a capture antibody.Applications for NFX1 Antibody (1D12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NFX1
Alternate Names
DKFZp779G2416, EC 6.3.2.-, MGC20369, NFX2transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, nuclear transcription factor, X-box binding 1
Entrez Gene IDs
4799 (Human)
Gene Symbol
NFX1
OMIM
603255 (Human)
Additional NFX1 Products
Product Documents for NFX1 Antibody (1D12)
Product Specific Notices for NFX1 Antibody (1D12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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