NFX1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49933
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1070 and 1120 of human Nuclear Transcription Factor, X-box Binding 1 using the numbering given in entry NP_002495.2 (GeneID 4799).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NFX1 Antibody
Western Blot: NFX1 Antibody [NBP1-49933]
Western Blot: NFX1 Antibody [NBP1-49933] - Detection of Human and Mouse NFX1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-NFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated NFX1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).Applications for NFX1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NFX1
Alternate Names
DKFZp779G2416, EC 6.3.2.-, MGC20369, NFX2transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, nuclear transcription factor, X-box binding 1
Gene Symbol
NFX1
Additional NFX1 Products
Product Documents for NFX1 Antibody
Product Specific Notices for NFX1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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