NFX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49933

Novus Biologicals, part of Bio-Techne
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NBP1-49933
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NFX1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1070 and 1120 of human Nuclear Transcription Factor, X-box Binding 1 using the numbering given in entry NP_002495.2 (GeneID 4799).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFX1 Antibody

Western Blot: NFX1 Antibody [NBP1-49933]

Western Blot: NFX1 Antibody [NBP1-49933]

Western Blot: NFX1 Antibody [NBP1-49933] - Detection of Human and Mouse NFX1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-NFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated NFX1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for NFX1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NFX1

MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.

Alternate Names

DKFZp779G2416, EC 6.3.2.-, MGC20369, NFX2transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, nuclear transcription factor, X-box binding 1

Gene Symbol

NFX1

Additional NFX1 Products

Product Documents for NFX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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