NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47965
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965]](https://resources.bio-techne.com/images/products/NGFR-TNFRSF16-p75NTR-Antibody-NGFR5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47965-img0002.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Baboon, Feline, Ferret, Monkey, Mouse (Negative), Rabbit, Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # NGFR5
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free
Immunogen
NGFR from A875 melanoma cells
Epitope
aa 1-160
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Cell surface and cytoplasmic
Specificity
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
Marker
Soft Tissue Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44874).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965]
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965] - Human Adrenal Gland stained with NGFR Mouse Monoclonal Antibody (NGFR5).![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965]](https://resources.bio-techne.com/images/products/NGFR-TNFRSF16-p75NTR-Antibody-NGFR5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47965-img0001.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965]")
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965]
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965] - Human Melanoma stained with NGFR Mouse Monoclonal Antibody (NGFR5).Applications for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: NGFR/TNFRSF16
Long Name
Nerve Growth Factor Receptor
Alternate Names
CD271, NGF R, p75 NTR, TNFRSF16
Gene Symbol
NGFR
Additional NGFR/TNFRSF16 Products
Product Documents for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free
Product Specific Notices for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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