NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47965PECY55
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Baboon, Feline, Ferret, Monkey, Mouse (Negative), Rabbit, Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # NGFR5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [PE/Cy5.5]
Immunogen
NGFR from A875 melanoma cells
Epitope
aa 1-160
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Cell surface and cytoplasmic
Specificity
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
Marker
Soft Tissue Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [PE/Cy5.5]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: NGFR/TNFRSF16
Long Name
Nerve Growth Factor Receptor
Alternate Names
CD271, NGF R, p75 NTR, TNFRSF16
Gene Symbol
NGFR
Additional NGFR/TNFRSF16 Products
Product Documents for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [PE/Cy5.5]
Product Specific Notices for NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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