Nicastrin Antibody (004)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89826
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826]](https://resources.bio-techne.com/images/products/Nicastrin-Antibody-004-Immunocytochemistry-Immunofluorescence-NBP2-89826-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 004
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Nicastrin (Accession#: NP_056146.1; Met1-Glu669).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Nicastrin Antibody (004)
Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826]
Immunocytochemistry/Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826] - Staining of Human NCSTN in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NCSTN monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.Applications for Nicastrin Antibody (004)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nicastrin
Alternate Names
APH2, NCSTN
Gene Symbol
NCSTN
Additional Nicastrin Products
Product Documents for Nicastrin Antibody (004)
Product Specific Notices for Nicastrin Antibody (004)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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