Nicastrin Antibody (019)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89828
Recombinant Monoclonal Antibody
![Western Blot: Nicastrin Antibody (019) [NBP2-89828]](https://resources.bio-techne.com/images/products/Nicastrin-Antibody-019-Western-Blot-NBP2-89828-img0001.jpg "Western Blot: Nicastrin Antibody (019) [NBP2-89828]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 019
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Nicastrin (Accession#: NP_056146.1; Met1-Glu669).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Nicastrin Antibody (019)
Western Blot: Nicastrin Antibody (019) [NBP2-89828]
Western Blot: Nicastrin Antibody (019) [NBP2-89828] - Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:78 kDa Observed band size:170 kDa (We are unsure as to the identity of these extra bands.)![Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828]](https://resources.bio-techne.com/images/products/Nicastrin-Antibody-019-Immunoprecipitation-NBP2-89828-img0002.jpg "Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828]")
Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828]
Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-NCSTN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NCSTN rabbit monoclonal antibody,at 1:150 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 78 kDa Observed band size: 130 kDaApplications for Nicastrin Antibody (019)
Application
Recommended Usage
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nicastrin
Alternate Names
APH2, NCSTN
Gene Symbol
NCSTN
Additional Nicastrin Products
Product Documents for Nicastrin Antibody (019)
Product Specific Notices for Nicastrin Antibody (019)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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