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Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00001137-M01

Novus Biologicals, part of Bio-Techne
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H00001137-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CHRNA4 (NP_000735, 29 a.a. ~ 128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HVETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNN

Specificity

CHRNA4 - cholinergic receptor, nicotinic, alpha 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3)

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3) [H00001137-M01]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3) [H00001137-M01]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3.) [H00001137-M01] - Detection against Immunogen (36.74 KDa) .

Applications for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 4/CHRNA4

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described.

Long Name

Nicotinic Acetylcholine Receptor alpha 4

Alternate Names

BFNC, CHRNA4, EBN1, ENFL1, nAChR alpha 4, nAchRa4, NACRA4, Nicotinic Acetylcholine Ra4

Entrez Gene IDs

1137 (Human)

Gene Symbol

CHRNA4

OMIM

118504 (Human)

UniProt

Additional Nicotinic Acetylcholine R alpha 4/CHRNA4 Products

Product Documents for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (4E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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