NLRP1/NALP1 Antibody

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148]

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE normal human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) epidermis, (B) esophagus (C) uterine cervix, and (D) thymus. Hematoxylin-eosin counterstain. In A-C, NALP1 expression is associated with differentiation in stratified epithelial of the skin, esophagus, and cervix.

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148]

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) bone marrow and (C) brain glioma core from a brain cancer microarray. (B) and (D) are higher magnifications of (A) and (C) respectively. Hemaxotylin-eosin counterstain.

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] -

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] - Gene expression & cellular distribution of NLRP1, NLRP3 & Aim2 sensors in the retina. (A) RNAscope analysis of NLRP1 (green dots, yellow arrows) & NLRP3 (white dots, white arrows) transcript abundance in normotensive control (NT control) & experimental (OHT) retinas at 12 h postinjury. (B) Aim2 transcript (red dots) abundance in normotensive control & experimental retinas. Co-staining for the Muller glia marker glutamine synthetase (GlSyn, green) is added to indicate cellular expression of Aim2 in the GCL layer (yellow arrows) & INL (white arrows) in control & 12 h post-OHT retinas. Bar, 25 μm on all panels. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30930743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] -

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] - OHT-induced upregulation of Aim2 inflammasome in the retina. (A) NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC neurons (RBPSM+) in the GCL. (B) Immunohistochemistry for Aim2 protein (red) & Muller glia marker (GlSyn, green). Arrows show colocalization with Muller glia; no colocalization is detected with astrocytes (GFAP, white) in both control & 24 h post-OHT retinas. (C) NLRP3 labeling (green) in the inner retina of naïve & control eyes localized only to blood capillaries (white arrowhead) & astrocytes (yellow arrows), but not to RGCs (RBPMS cells). At 24 h post-injury the labeling in large cells in the GCL & INL (white arrows) are observed. (D) The NLRP1and NLRP3 proteins localized to distinct cell types in the GCL of naïve & NT control retinas: NLRP3 (green) is only expressed in blood vessels (white arrowheads). Twenty four hours after OHT, NLRP3 colocalizes with NLRP1 in RGCs (yellow arrows) & to RBPMS- cells (white arrows). Blue arrowheads denote labeling in RGC axons & dendrites. Bar, 25 μm on all panels. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30930743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.