Notch-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2414

Novus Biologicals, part of Bio-Techne
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NB100-2414
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Notch-3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QLGPQPEVTPKRQ corresponding to C-Terminus according to NP_000426.2.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Notch-3 Antibody

Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414]

Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414]

Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414] - Staining of paraffin embedded Human Placenta. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Notch-3 Antibody [NB100-2414]

Flow Cytometry: Notch-3 Antibody [NB100-2414]

Flow Cytometry: Notch-3 Antibody [NB100-2414] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414]

Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414]

Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414] - Staining of paraffin embedded Human Hippocampus. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Notch-3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:16000
Application Notes
Notch-3 Antibody validated for IP from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NB100-2414 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Notch-3

The NOTCH3 gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays

Alternate Names

Notch3

Entrez Gene IDs

4854 (Human)

Gene Symbol

NOTCH3

UniProt

Q9UM47

Additional Notch-3 Products

Product Documents for Notch-3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Notch-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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