Notch-3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93550
![Immunohistochemistry-Frozen: Notch-3 Antibody - BSA Free [NB100-93550]](https://resources.bio-techne.com/images/products/Notch3-Antibody-Immunohistochemistry-Frozen-NB100-93550-img0002.jpg "Immunohistochemistry-Frozen: Notch-3 Antibody - BSA Free [NB100-93550]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide representing the C terminal region of the human NOTCH homolog 3 (NOTCH3) protein.
Reactivity Notes
This antibody is reactive against Human
Specificity
Notch 3 homolog
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
243.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Notch-3 Antibody - BSA Free
Immunohistochemistry-Frozen: Notch-3 Antibody - BSA Free [NB100-93550]
Immunohistochemistry-Frozen: Notch3 Antibody [NB100-93550] - Notch 3 homolog staining was performed on 10 micron crysostat sections of rat brain fixed with 4% formaldehyde by transcardial perfusion. 1:250 dilution of Notch 3 antibody was employed and incubation was performed overnight at 4C. Stained tissues were mounted under iBright mounting media.Applications for Notch-3 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
This Notch3 antibody is useful for Western Blot, ELISA and immunohistochemistry-Frozen applications. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 26341090).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Notch-3
Additional Notch-3 Products
Product Documents for Notch-3 Antibody - BSA Free
Product Specific Notices for Notch-3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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