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Orexin R2/HCRTR2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74579

Novus Biologicals, part of Bio-Techne
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NB100-74579

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence SGTKLEDSPPCRN corresponding to N-Terminus according to NP_001517.1.

Epitope

SGTKLEDSPPCRN

Localization

Isoform 1 and isoform 4: Cell membrane; single pass type I membrane protein. Isoform 2 and isoform 3: Secreted protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Orexin R2/HCRTR2 Antibody

Western Blot: Orexin R2/HCRTR2 Antibody [NB100-74579]

Western Blot: Orexin R2/HCRTR2 Antibody [NB100-74579]

Western Blot: Orexin R2/HCRTR2 Antibody [NB100-74579] - (1.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Orexin R2/HCRTR2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human brain (cerebral cortex, hippocampus and substantia nigra) lysates (calculated MW of 50.7 kDa band according to NP_001517.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Orexin R2/HCRTR2

HCRTR2 is a G-protein coupled receptor expressed exclusively in the brain. It has 64% identity with HCRTR1. HCRTR2 binds both orexin A and orexin B neuropeptides. HCRTR2 is involved in the central feedback mechanism that regulates feeding behaviour.

Long Name

Hypocretin Receptor Type 2

Alternate Names

HCRTR2, Orexin Receptor 2, OrexinR2, OX2R

Entrez Gene IDs

3062 (Human); 25605 (Rat)

Gene Symbol

HCRTR2

Additional Orexin R2/HCRTR2 Products

Product Documents for Orexin R2/HCRTR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Orexin R2/HCRTR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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