Ovalbumin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-922

Novus Biologicals, part of Bio-Techne
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NB600-922SS
NB600-922-0.1mg
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Key Product Details

Species Reactivity

Validated:

Chicken

Cited:

Human, Avian - Chicken, Virus

Applications

Validated:

ELISA, Immunohistochemistry, Single Cell Western, Western Blot

Cited:

ELISA, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ovalbumin Antibodies »

Product Specifications

Immunogen

Ovalbumin [Hen Egg White] (Uniprot: P01012)

Reactivity Notes

Cross reactivity against Ovalbumin from other sources is unknown

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Ovalbumin Antibody

Western Blot: Ovalbumin Antibody [NB600-922]

Western Blot: Ovalbumin Antibody [NB600-922]

Western Blot: Ovalbumin Antibody [NB600-922] - Lane 1: Reduced Ovalbumin. Load: 50 ng. Primary antibody: Ovalbumin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin.
Western Blot: Ovalbumin Antibody [NB600-922]

Western Blot: Ovalbumin Antibody [NB600-922]

Western Blot: Ovalbumin Antibody [NB600-922] - Western blot detection of Ovalbumin from hen egg white using NB600-922 at 1:10,000. The blot was blocked ON at 4 degrees C in 3% BSA. The secondary antibody used was a DyLight 649 Conjugated Goat anti-rabbit IgG (H&L) diluted 1:20,000 and incubated 1 hr RT in blocking buffer for fluorescent western blotting.
Western Blot: Ovalbumin Antibody [NB600-922]

Western Blot: Ovalbumin Antibody [NB600-922]

Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
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Applications for Ovalbumin Antibody

Application
Recommended Usage

ELISA

1:5000-1:20000

Single Cell Western

1:10

Western Blot

1:500-1:2000
Application Notes
SCW is validated using murine sarcoma cells

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ovalbumin

Anti Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.

Alternate Names

Allergen Gal d 2, CAP 2, CAP2, Cytoplasmic antiproteinase 2, Egg albumin, Gal d II, Hen egg white, PI8, Plakalbumin, Protease inhibitor 8, Serpin B8, serpin peptidase inhibitor clade B member 8, SERPINB14, SERPINB8

Gene Symbol

OVAL

UniProt

P01012 (Chicken)

Additional Ovalbumin Products

Product Documents for Ovalbumin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ovalbumin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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