Ovalbumin Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-924
Key Product Details
Species Reactivity
Chicken
Applications
Dot Blot, ELISA, Western Blot
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Ovalbumin [Hen Egg White] (Uniprot: P01012)
Reactivity Notes
Cross reactivity against Ovalbumin from other sources is unknown
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]
Scientific Data Images for Ovalbumin Antibody [Biotin]
Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: ~1 ug Ovalbumin protein reduced. Lane 2: 0.25 ug Ovalbumin protein reduced. Lane 3: ~1 ug Ovalbumin protein non-reduced. Lane 4: 0.25 ug Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block buffer overnight at 4C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin.
Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
Applications for Ovalbumin Antibody [Biotin]
Application
Recommended Usage
ELISA
1:30000
Western Blot
1:500-1:5000
Application Notes
This antibody Biotin Conjugated is suitable for both ELISA, and for western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Ovalbumin
Alternate Names
Allergen Gal d 2, CAP 2, CAP2, Cytoplasmic antiproteinase 2, Egg albumin, Gal d II, Hen egg white, PI8, Plakalbumin, Protease inhibitor 8, Serpin B8, serpin peptidase inhibitor clade B member 8, SERPINB14, SERPINB8
Gene Symbol
OVAL
UniProt
Additional Ovalbumin Products
Product Documents for Ovalbumin Antibody [Biotin]
Product Specific Notices for Ovalbumin Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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