P-Selectin/CD62P Antibody (AK-4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43392

Clone AK-4 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-43392-0.025mg
NBP1-43392-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AK-4

Format

BSA Free

Concentration

0.5 mg/ml

View all P-Selectin/CD62P Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD62P.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for P-Selectin/CD62P Antibody (AK-4) - BSA Free

Flow Cytometry: P-Selectin/CD62P Antibody (AK-4) - BSA Free [NBP1-43392]

Flow Cytometry: P-Selectin/CD62P Antibody (AK-4) - BSA Free [NBP1-43392]

Flow Cytometry: P-Selectin/CD62P Antibody (AK-4) [NBP1-43392] - Staining of thrombin-activated human peripheral blood platelets with 0.5 ug of Mouse IgG1 kappa Isotype Control Purifed (open histogram) or 0.5 ug of Anti-Human CD62P (P-Selectin) Purified (filled histogram) followed by Anti-Mouse IgG FITC. Total viable cells were used for analysis.

Applications for P-Selectin/CD62P Antibody (AK-4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
This AK-4 antibody has been tested by flow cytometric analysis of resting and thrombin-activated human platelets. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: P-Selectin/CD62P

The AK-4 monoclonal antibody reacts with human CD62P, a 140 kDa transmembrane glycoprotein also known as P-Selectin. Upon activation, CD62P is rapidly transported from its cytoplasmic stores in the Weibel-Palade bodies of endothelial cells or the alpha-granules of platelets to the surface. CD62P initiates the adhesive interaction between endothelial cells and neutrophils and monocytes during the inflammatory reaction and is also involved in the interaction of platelets with monocytes and neutrophils.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (AK-4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for P-Selectin/CD62P Antibody (AK-4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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