P-Selectin/CD62P Antibody (Psel.KO.2.7)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65392
![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]](https://resources.bio-techne.com/images/products/P-Selectin-CD62P-Antibody-Psel-KO-2-7-Flow-Cytometry-NB100-65392-img0008.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]")
![Immunocytochemistry/ Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]](https://resources.bio-techne.com/images/products/P-Selectin-CD62P-Antibody-Psel-KO-2-7-Immunohistochemistry-NB100-65392-img0015.jpg "Immunocytochemistry/ Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Equine
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # Psel.KO.2.7
Concentration
1.0 mg/ml
Product Specifications
Immunogen
P-selectin transfected 300.19 cells
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Localization
Membrane; single pass type I membrane protein.
Specificity
NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for P-Selectin/CD62P Antibody (Psel.KO.2.7)
Immunocytochemistry/ Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]
Immunocytochemistry/Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Alignment of live-cell calcium response behavior with PTH abundance in a parathyroid adenoma. (A) Prestimulation baseline flux activity at time 0; (B) same field of view at 2 minutes after stimulation with 2 mM calcium; (C) same field after fixation and immunofluorescent staining for PTH (red); (D) image overlay of (B) and (C). Flux images (A, B) are single timepoint frames from the same time-ordered image stack. Anti-PTH reactivity is visualized by an AlexaFluor555 secondary antibody. Magnification = 200X. Image using the Allophycocyanin format of this antibody.![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]](https://resources.bio-techne.com/images/products/P-Selectin-CD62P-Antibody-Psel-KO-2-7-Flow-Cytometry-NB100-65392-img0013.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]")
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Thrombin stimulated equine platelets (solid line) and unstimulated platelets (dashed line) labeled with anti-CD62P (Psel.KO.2.7) compared to equine platelets labeled with murine IgG-DyLight 488 isotype control (gray fill).![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]](https://resources.bio-techne.com/images/products/P-Selectin-CD62P-Antibody-Psel-KO-2-7-Flow-Cytometry-NB100-65392-img0007.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]")
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]](https://resources.bio-techne.com/images/products/P-Selectin-CD62P-Antibody-Psel-KO-2-7-Flow-Cytometry-NB100-65392-img0009.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]")
![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]](https://resources.bio-techne.com/images/products/P-Selectin-CD62P-Antibody-Psel-KO-2-7-Flow-Cytometry-NB100-65392-img0012.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]")
Applications for P-Selectin/CD62P Antibody (Psel.KO.2.7)
Application
Recommended Usage
Flow Cytometry
1:25-1:50
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. IHC-Frozen use reported in literature (see Massaguer, A. et al, 2000). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31237986).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P-Selectin/CD62P
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Entrez Gene IDs
6403 (Human)
Gene Symbol
SELP
UniProt
Additional P-Selectin/CD62P Products
Product Documents for P-Selectin/CD62P Antibody (Psel.KO.2.7)
Product Specific Notices for P-Selectin/CD62P Antibody (Psel.KO.2.7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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