P2X7/P2RX7 Antibody (Hano43)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40894

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # Hano43

Concentration

1 mg/ml

View all P2X7/P2RX7 Antibodies »

Product Specifications

Immunogen

A P2X7-expression construct and a final boost with P2X7-transfected HEK cells

Specificity

This antibody recognises the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for P2X7/P2RX7 Antibody (Hano43)

Flow Cytometry: P2X7/P2RX7 Antibody (Hano43) - BSA Free [NBP1-40894]

Flow Cytometry: P2X7/P2RX7 Antibody (Hano43) - BSA Free [NBP1-40894]

Flow Cytometry: P2X7/P2RX7 Antibody (Hano43) [NBP1-40894] - Flow Cytometry: P2X7/P2RX7 Antibody (Hano43) [Alexa Fluor® 647] [NBP1-40894AF647] - Mouse dendritic cells (BMDCs) stained for CD11c and P2X7. Image from verified customer review. Image using the Alexa Fluor 647 form of this antibody.

Applications for P2X7/P2RX7 Antibody (Hano43)

Application
Recommended Usage

Flow Cytometry

1:25-1:200

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P2X7/P2RX7

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq]

Long Name

P2X Purinergic Receptor 7

Alternate Names

P2RX7

Entrez Gene IDs

18439 (Mouse)

Gene Symbol

P2RX7

UniProt

Q9Z1M0 (Mouse)

Additional P2X7/P2RX7 Products

Product Documents for P2X7/P2RX7 Antibody (Hano43)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for P2X7/P2RX7 Antibody (Hano43)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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