P2X7/P2RX7 Antibody (Hano43) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40894CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
CoraFluor 1
Antibody Source
Monoclonal Rat IgG2B Clone # Hano43
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A P2X7-expression construct and a final boost with P2X7-transfected HEK cells
Reactivity Notes
Reacts with Mouse.
Specificity
This antibody recognises the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for P2X7/P2RX7 Antibody (Hano43) [CoraFluor™ 1]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: P2X7/P2RX7
Long Name
P2X Purinergic Receptor 7
Alternate Names
P2RX7
Gene Symbol
P2RX7
Additional P2X7/P2RX7 Products
Product Documents for P2X7/P2RX7 Antibody (Hano43) [CoraFluor™ 1]
Product Specific Notices for P2X7/P2RX7 Antibody (Hano43) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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