PAK3 Antibody (3A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005063-M08

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PAK3 Antibodies »

Product Specifications

Immunogen

PAK3 (NP_002569, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSDGLDNEEKPPAPPLRMNSNNRDSSALNHSSKPLPMAPEEKNKKARLRSIFPGGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

PAK3 - p21 (CDKN1A)-activated kinase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PAK3 Antibody (3A12)

Western Blot: PAK3 Antibody (3A12) [H00005063-M08]

Western Blot: PAK3 Antibody (3A12) [H00005063-M08]

Western Blot: PAK3 Antibody (3A12) [H00005063-M08] - Analysis of PAK3 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: PAK3 Antibody (3A12) [H00005063-M08]

Immunocytochemistry/ Immunofluorescence: PAK3 Antibody (3A12) [H00005063-M08]

Immunocytochemistry/Immunofluorescence: PAK3 Antibody (3A12) [H00005063-M08] - Analysis of monoclonal antibody to PAK3 on NIH/3T3 cell. Antibody concentration 10 ug/ml

Applications for PAK3 Antibody (3A12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAK3

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified.

Long Name

p21-activated Kinase 3

Alternate Names

beta-PAK, MRX30, MRX47, OPHN3

Entrez Gene IDs

5063 (Human)

Gene Symbol

PAK3

OMIM

300142 (Human)

UniProt

NP_002569 (Human)

Additional PAK3 Products

Product Documents for PAK3 Antibody (3A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PAK3 Antibody (3A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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