PAK4 Antibody (OTI1C7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73231
![Western Blot: PAK4 Antibody (OTI1C7)Azide and BSA Free [NBP2-73231]](https://resources.bio-techne.com/images/products/PAK4-Antibody-OTI1C7-Azide-and-BSA-Free-Western-Blot-NBP2-73231-img0001.jpg "Western Blot: PAK4 Antibody (OTI1C7)Azide and BSA Free [NBP2-73231]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1C7
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-308 of human PAK4(NP_005875) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
63.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PAK4 Antibody (OTI1C7) - Azide and BSA Free
Western Blot: PAK4 Antibody (OTI1C7)Azide and BSA Free [NBP2-73231]
Western Blot: PAK4 Antibody (OTI1C7) - Azide and BSA Free [NBP2-73231] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4.Applications for PAK4 Antibody (OTI1C7) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PAK4
Long Name
p21/Cdc42/Rac1-activated Kinase 4
Alternate Names
EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4
Gene Symbol
PAK4
Additional PAK4 Products
Product Documents for PAK4 Antibody (OTI1C7) - Azide and BSA Free
Product Specific Notices for PAK4 Antibody (OTI1C7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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