Pax7 Antibody (SPM613)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44589
![Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) [NBP2-44589]](https://resources.bio-techne.com/images/products/Pax7-Antibody-SPM613-Immunohistochemistry-Paraffin-NBP2-44589-img0001.jpg "Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) [NBP2-44589]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM613
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:32019766).
Localization
Nuclear
Marker
Rhabdomyosarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47923)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Pax7 Antibody (SPM613)
Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) [NBP2-44589]
Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) [NBP2-44589] - Human Rhabdomyosarcoma stained with PAX7 Monoclonal Antibody (SPM613).Applications for Pax7 Antibody (SPM613)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID: 32019766).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Pax7
Additional Pax7 Products
Product Documents for Pax7 Antibody (SPM613)
Product Specific Notices for Pax7 Antibody (SPM613)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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