Pax7 Antibody (SPM613) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48249
![Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) - IHC-Prediluted [NBP2-48249]](https://resources.bio-techne.com/images/products/Pax7-Antibody-SPM613-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48249-img0001.jpg "Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) - IHC-Prediluted [NBP2-48249]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM613
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:32019766).
Localization
Nuclear
Marker
Rhabdomyosarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Pax7 Antibody (SPM613) - IHC-Prediluted
Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) - IHC-Prediluted [NBP2-48249]
Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) - IHC-Prediluted [NBP2-48249] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with PAX7 Monoclonal Antibody (SPM613).Applications for Pax7 Antibody (SPM613) - IHC-Prediluted
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID: 32019766).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Pax7
Long Name
Paired Box Gene 7
Alternate Names
HUP1, RMS2
Gene Symbol
PAX7
Additional Pax7 Products
Product Documents for Pax7 Antibody (SPM613) - IHC-Prediluted
Product Specific Notices for Pax7 Antibody (SPM613) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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