PD-1 Antibody (PDCD1/4231R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20955
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # PDCD1/4231R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment corresponding to extracellular domain of human PD-1 protein (exact sequence is proprietary)
Localization
Cell surface. Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Human tonsil, lymph node or appendix.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for PD-1 Antibody (PDCD1/4231R) - Azide and BSA Free
PD-1 Antibody (PDCD1/4231R) - Azide and BSA Free
Formalin-fixed, paraffin-embedded human lymph node stained with PD1 (CD279) Recombinant Rabbit Monoclonal Antibody (PDCD1/4231R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
PD-1 Antibody (PDCD1/4231R) - Azide and BSA Free
Formalin-fixed, paraffin-embedded human tonsil stained with PD1 (CD279) Recombinant Rabbit Monoclonal Antibody (PDCD1/4231R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for PD-1 Antibody (PDCD1/4231R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PD-1
Long Name
Programmed Death-1
Alternate Names
CD279, PD1, PDCD1, SLEB2
Gene Symbol
PDCD1
Additional PD-1 Products
Product Documents for PD-1 Antibody (PDCD1/4231R) - Azide and BSA Free
Product Specific Notices for PD-1 Antibody (PDCD1/4231R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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