PDE4B Antibody (OTI1D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01171

Novus Biologicals, part of Bio-Techne
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NBP2-01171
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-73292AF350
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Alexa Fluor 405
NBP2-73292AF405
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Alexa Fluor 488
NBP2-73292AF488
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Alexa Fluor 532
NBP2-73292AF532
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Alexa Fluor 594
NBP2-73292AF594
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Alexa Fluor 647
NBP2-73292AF647
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Alexa Fluor 700
NBP2-73292AF700
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Alexa Fluor 750
NBP2-73292AF750
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Allophycocyanin
NBP2-73292APC
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Allophycocyanin/Cy7
NBP2-73292APCCY7
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Biotin
NBP2-73292B
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CoraFluor 1
NBP2-73292CL1
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DyLight 350
NBP2-73292UV
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DyLight 405
NBP2-73292V
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DyLight 488
NBP2-73292G
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DyLight 550
NBP2-73292R
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DyLight 594
NBP2-73292DL594
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DyLight 650
NBP2-73292C
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DyLight 680
NBP2-73292FR
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DyLight 755
NBP2-73292IR
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FITC
NBP2-73292F
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HRP
NBP2-73292H
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Janelia Fluor 525
NBP2-73292JF525
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Janelia Fluor 549
NBP2-73292JF549
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Janelia Fluor 585
NBP2-73292JF585
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Janelia Fluor 635
NBP2-73292JF635
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Janelia Fluor 646
NBP2-73292JF646
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Janelia Fluor 669
NBP2-73292JF669
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mFluor Violet 450 SE
NBP2-73292MFV450
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mFluor Violet 500 SE
NBP2-73292MFV500
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mFluor Violet 610 SE
NBP2-73292MFV610
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PE
NBP2-73292PE
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PE/Atto594
NBP2-73292PEATT594
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PE/Cy5.5
NBP2-73292PECY55
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PE/Cy7
NBP2-73292PECY7
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PerCP
NBP2-73292PCP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D12

Concentration

0.62 mg/ml

View all PDE4B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDE4B(NP_002591) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

83.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE4B Antibody (OTI1D12)

Western Blot: PDE4B Antibody (1D12) [NBP2-01171] Analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunocytochemistry/ Immunofluorescence: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunocytochemistry/Immunofluorescence: PDE4B Antibody (1D12) [NBP2-01171] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Human prostate tissue using anti-PDE4B mouse monoclonal antibody.
View 9 more images

Applications for PDE4B Antibody (OTI1D12)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.62 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE4B

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Long Name

cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Alternate Names

DPDE4, PDE32

Entrez Gene IDs

5142 (Human)

Gene Symbol

PDE4B

Additional PDE4B Products

Product Documents for PDE4B Antibody (OTI1D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDE4B Antibody (OTI1D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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