PDGF R beta Antibody (APB5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43349
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Protein Array
Cited:
Flow Cytometry, Immunohistochemistry-Frozen, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # APB5
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was PDGF receptor beta.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for PDGF R beta Antibody (APB5) - BSA Free
Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]
Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Mouse brain pericytes - IgG (left) and PDGFRB (right). Image from verified customer review.Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]
Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (cat. 14-4321) (open histogram) or 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Purified (filled histogram) followed by Anti-Rat IgG Biotin (cat. 13-4813) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]
Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Analysis using the Biotin conjugate of NBP1-43349. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).Applications for PDGF R beta Antibody (APB5) - BSA Free
Application
Recommended Usage
Flow Cytometry
< 1.0 ug/10^6 cells
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Application Notes
The APB5 antibody has been tested by flow cytometric analysis of NIH-3T3 cells. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Use in Protein Array reported in secitific publication PMID: 32499572
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP1-43349 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PDGF R beta
Long Name
Platelet-derived Growth Factor Receptor beta
Alternate Names
CD140b, PDGFRB
Entrez Gene IDs
18596 (Mouse)
Gene Symbol
PDGFRB
UniProt
Additional PDGF R beta Products
Product Documents for PDGF R beta Antibody (APB5) - BSA Free
Product Specific Notices for PDGF R beta Antibody (APB5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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