PDGF R beta [p Tyr1009] Antibody (JE46-72)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32785
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE46-72
Concentration
1 mg/ml
Product Summary for PDGF R beta [p Tyr1009] Antibody (JE46-72)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr1009 of human PDGF R beta. (Uniprot: P09619)
Modification
p Tyr 1009
Localization
Cell membrane, Cytoplasmic vesicle, Lysosome lumen.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PDGF R beta [p Tyr1009] Antibody (JE46-72)
![PDGF R beta [p Tyr1009] Antibody (JE46-72)](https://resources.bio-techne.com/images/products/nbp3-32785_rabbit-pdgf-r-beta-p-tyr1009-mab-je46-72-288202412461643.jpg "Western Blot: PDGF R beta [p Tyr1009] Antibody (JE46-72) [NBP3-32785]")
Western Blot: PDGF R beta [p Tyr1009] Antibody (JE46-72) [NBP3-32785]
Western Blot: PDGF R beta [p Tyr1009] Antibody (JE46-72) [NBP3-32785] - Western blot analysis of PDGF R beta on different lysates with Rabbit anti-PDGF R beta antibody (NBP3-32785) at 1/1,000 dilution.Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 124 kDa
Observed band size: 200 kDa (Glycosylation)
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32785) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for PDGF R beta [p Tyr1009] Antibody (JE46-72)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PDGF R beta
Long Name
Platelet-derived Growth Factor Receptor beta
Alternate Names
CD140b, PDGFRB
Gene Symbol
PDGFRB
Additional PDGF R beta Products
Product Documents for PDGF R beta [p Tyr1009] Antibody (JE46-72)
Product Specific Notices for PDGF R beta [p Tyr1009] Antibody (JE46-72)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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