PDK-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44132
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PDK-1 Antibody
Immunogen
This affinity purified PDK-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human PDK-1 protein. (Uniprot: Q15118)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with PDK-1 protein from mouse, rat and dog based on 92% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against human PDK-1. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for PDK-1 Antibody
![Western Blot: PDK-1 Antibody [NBP2-44132]](https://resources.bio-techne.com/images/products/PDPK1-Antibody-Western-Blot-NBP2-44132-img0001.jpg "Western Blot: PDK-1 Antibody [NBP2-44132]")
Western Blot: PDK-1 Antibody [NBP2-44132]
Western Blot: PDPK1 Antibody [NBP2-44132] - Detection of myc-tagged human PDK-1 at 60kDa in 10 Ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 C with theApplications for PDK-1 Antibody
Application
Recommended Usage
ELISA
1:100000
Immunohistochemistry
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~60 kDa in size corresponding to human PDK-1 protein by western blotting in the appropriate cell lysate or extract. Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation. Reactivity in other immunoassays is unknown.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: PDK-1
Long Name
Phosphoinositide Dependent Kinase-1
Alternate Names
PDK1, PDPK1, PKB kinase
Gene Symbol
PDPK1
UniProt
Additional PDK-1 Products
Product Documents for PDK-1 Antibody
Product Specific Notices for PDK-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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