PDK-1 [p Tyr9] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23292

Novus Biologicals, part of Bio-Techne
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NBP3-23292
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PDK-1 Antibodies »

Product Summary for PDK-1 [p Tyr9] Antibody

Immunogen

Phospho-PDK-1 (Tyr-9) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 9 in human PDK-1. This sequence is highly conserved in rat and mouse PDK-1. [UniProt# O15530]

Modification

p Tyr9

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDK-1 (Tyr-9) peptide. The purified antibody detects a 65 kDa protein corresponding to the apparent molecular mass of PDK-1 on SDS-PAGE immunoblots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells. PDK1 (Tyr-9) reactivity was also observed in adult mouse brain.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PDK-1 [p Tyr9] Antibody

PDK-1 [p Tyr9] Antibody

Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] -

Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Western blot image of A431 cells stimulated with pervanadate (lanes 1 and 3). The blots were then treated with akaline phosphatase (lanes 2 & 4) then probed with anti-PDK-1 (C-terminal region) mouse monoclonal antibody or anti-PDK-1 (Tyr-9) (NBP3-23292) rabbit polyclonal antibody.
PDK-1 [p Tyr9] Antibody

Immunocytochemistry/Immunofluorescence: PDK-1 [p Tyr9] Antibody [NBP3-23292] -

Immunocytochemistry/Immunofluorescence: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK-1 (C-terminal Region) (NBP3-23291), rabbit polyclonal PDK-1 (Tyr-9) (NBP3-23292), and PDK-1 (N-terminus) (NBP3-23290) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Applications for PDK-1 [p Tyr9] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PDK-1

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Gene Symbol

PDPK1

Additional PDK-1 Products

Product Documents for PDK-1 [p Tyr9] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDK-1 [p Tyr9] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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