PGC1 alpha Antibody (1G8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37562

Novus Biologicals, part of Bio-Techne
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NBP2-37562
NBP2-37562-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1G8

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all PGC1 alpha Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PGC1 alpha expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGC1 alpha Antibody (1G8) - BSA Free

Western Blot: PGC1 alpha Antibody (1G8)BSA Free [NBP2-37562]

Western Blot: PGC1 alpha Antibody (1G8)BSA Free [NBP2-37562]

Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against HEK293 (1) and PGC-1alpha-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: PGC1 alpha Antibody (1G8)BSA Free [NBP2-37562]

Western Blot: PGC1 alpha Antibody (1G8)BSA Free [NBP2-37562]

Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against human PGC-1alpha (AA: 570-798) recombinant protein. Expected molecular weight is 70 KDa.
ELISA: PGC1 alpha Antibody (1G8) - BSA Free [NBP2-37562]

ELISA: PGC1 alpha Antibody (1G8) - BSA Free [NBP2-37562]

ELISA: PGC1 alpha Antibody (1G8) [NBP2-37562] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PGC1 alpha Antibody (1G8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGC1 alpha

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O., ... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Alternate Names

LEM6, PPARGC1A

Gene Symbol

PPARGC1A

Additional PGC1 alpha Products

Product Documents for PGC1 alpha Antibody (1G8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PGC1 alpha Antibody (1G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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