PGC1 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1750

Novus Biologicals, part of Bio-Techne
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NB100-1750
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.25 mg/ml

View all PGC1 alpha Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this PGC1 alpha Antibody maps to a region between residue 250 and 300 of human peroxisome proliferative activated receptor, gamma, coactivator 1, alpha using the numbering given in SwissProt entry Q9UBK2 (Gene ID 10891).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23892277).

Specificity

The epitope recognized by PGC1 alpha Antibody maps to a region between residue 250 and 300 of human PGC1 alpha using the numbering given in SwissProt entry Q9UBK2 (Gene ID: 10891).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGC1 alpha Antibody

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750]

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750]

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human colon adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750]

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750]

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human stomach adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB

Applications for PGC1 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500
Application Notes
Use in WB reported in scientific literature (PMID: 27875805). Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PGC1 alpha

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O., ... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Alternate Names

LEM6, PPARGC1A

Entrez Gene IDs

10891 (Human)

Gene Symbol

PPARGC1A

UniProt

Q9UBK2 (Human)

Additional PGC1 alpha Products

Product Documents for PGC1 alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PGC1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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