Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

Phospho-PDGF R beta ELISA Using APM1676 as Detection Antibody.

Detection of Phospho-Tyrosine by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

Applications for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

Application

Recommended Usage

ELISA

Western Blot

1:1000 dilution
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

For research use only

Citations
Reviews

Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # APM1676

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all Phospho-Tyrosine Antibodies »

Product Specifications

Specificity