Phospho-Tyrosine Antibody

Detection of Phospho-Tyrosine by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Detection of Human Phospho-Tyrosine by Simple Western^TM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 15 minutes, loaded at 0.2 mg/mL. Tyrosine-phosphorylated proteins were detected (as indicated) using 50 µg/mL of Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Phospho-Tyrosine in A431 Human Cell Line.

Phospho-Tyrosine was detected in immersion fixed A431 human epithelial carcinoma cell line treated with 50 ng/mL Recombinant Human EGF (left panel; Catalog # 236-EG) or untreated (right panel) using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.