PIF1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97931

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.16 mg/ml

View all PIF1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PIF1 protein (between residues 400-500) [UniProt Q9H611]

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIF1 Antibody - BSA Free

Immunohistochemistry: PIF1 Antibody - BSA Free [NBP1-97931]

Immunohistochemistry: PIF1 Antibody - BSA Free [NBP1-97931]

Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.

Applications for PIF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:300

Immunohistochemistry-Paraffin

1:300
Application Notes
In IHC-paraffin embedded sections, staining was observed in the nucleus of mouse brain tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot or ICC/IF.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.16 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIF1

PIF1 (pre-implantation factor, PIF1 5'-to-3' DNA helicase homolog, PIF, PIF1/RRM3 DNA helicase-like protein, C15orf20) is a member of the helicase family associated with embryo association with the uterus. PIF1 is also a DNA dependent ATPase that negatively regulates telomerase, the reverse transcriptase that affects telomere length (PMID 16522649). PIF1 is expressed in the nucleus in the late S/G2 phase and is carefully regulated by the cell cycle. In human tumor cells, the deletion of the PIF1 gene reduces the survival of both the p53-deficient and p53-proficient cells by initiating apoptosis by blocking S-phase entry and progression (PMID 21616935). Acting as a G4 resolvase, PIF1 prevents the formation of G4 (G-quadruplex) structures by stabilizing DNA sequences (PMID 20524933).

Alternate Names

C15orf20, PIF, PIF1 5-to-3 Prime DNA helicase homolog, RRM3 DNA helicase-like protein, PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae), PIF1 DNA helicase, PIF1/RRM3 DNA helicase-like protein

Entrez Gene IDs

80119 (Human)

Gene Symbol

PIF1

UniProt

Q9H611 (Human)

Additional PIF1 Products

Product Documents for PIF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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