Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PKR Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR. Amino Acid Squence: TVWKKSPEKNERHT

Specificity

Human PKR has 2 isoforms, including isoform 1 (551aa, 62.1kD) and isoform S (510aa, 57.4kD. This antibody detects both human isoforms. Mouse PKR has 1 isoform (515aa, 58.3kD). Rat PKR has only one isoform identified so far (513aa, 58.3kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKR Antibody - BSA Free

PKR Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PKR Antibody - BSA Free [NBP1-77266] -

Immunocytochemistry/ Immunofluorescence: PKR Antibody - BSA Free [NBP1-77266] - Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Western Blot: PKR AntibodyBSA Free [NBP1-77266]

Western Blot: PKR AntibodyBSA Free [NBP1-77266]

Western Blot: PKR Antibody [NBP1-77266] - WB Validation of PKR in Human Cell Lines. Antibodies: PKR. Secondary: Goat anti-rabbit IgG HRP conjugate.
Western Blot: PKR AntibodyBSA Free [NBP1-77266]

Western Blot: PKR AntibodyBSA Free [NBP1-77266]

Western Blot: PKR Antibody [NBP1-77266] - IAV via Protein Expression Profile in Cell Lines. Antibodies PKR and beta-actin. Secondary: Goat anti-rabbit IgG HRP conjugate.
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Applications for PKR Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5-5 ug/ml

Immunohistochemistry-Paraffin

2.5-5 ug/ml

Western Blot

1 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-77266 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKR

The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

Long Name

Protein Kinase R

Alternate Names

EIF2AK2, P68 Kinase, PPP1R83, PRKR, Protein Kinase R

Gene Symbol

EIF2AK2

UniProt

AAP57628 (Human)

Additional PKR Products

Product Documents for PKR Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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