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PMS2 Antibody (163C1251) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56554

Novus Biologicals, part of Bio-Techne
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NB100-56554SS
NB100-56554

Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 163C1251

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 623-639 SSLAKRIKQLHHEAQQS of human PMS2; GenBank No. NP_000526.1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PMS2 Antibody (163C1251) - BSA Free

Western Blot: PMS2 Antibody (163C1251)BSA Free [NB100-56554]

Western Blot: PMS2 Antibody (163C1251)BSA Free [NB100-56554]

Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.
Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) - BSA Free [NB100-56554]

Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) - BSA Free [NB100-56554]

Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using PMS2 antibody at 5 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PMS2 was observed. Staining was performed by Histowiz.

Applications for PMS2 Antibody (163C1251) - BSA Free

Application
Recommended Usage

Flow Cytometry

1ug per 10^6 cells

Immunohistochemistry

2-5ug/ml

Immunohistochemistry-Paraffin

2-5ug/ml

Western Blot

1-2 ug/ml
Application Notes
An approx. 85 kDa band should be observed. This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PMS2

PMS1 protein homologue 2 (PMS2) is a DNA mismatch repair protein. Defects in PMS2 are a cause of hereditary non-polyposis cancer (HNPCC). Human PMS2 is an amino acid protein of 862 amino acids.

Alternate Names

DNA mismatch repair protein PMS2, EC 3.1, H_DJ0042M02.9, HNPCC4postmeiotic segregation increased (S. cerevisiae) 2, mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2 (S. cerevisiae), PMSL2PMS2CL

Entrez Gene IDs

5395 (Human); 18861 (Mouse)

Gene Symbol

PMS2

UniProt

Additional PMS2 Products

Product Documents for PMS2 Antibody (163C1251) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PMS2 Antibody (163C1251) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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