Podocalyxin Like Antibody (3D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25219

Novus Biologicals, part of Bio-Techne
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NBP2-25219
NBP2-25219-20ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D3

Concentration

0.2 mg/ml

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27771514).

Localization

Cell surface and Cytoplasmic

Specificity

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Marker

Hematopoietic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33108)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Podocalyxin Like Antibody (3D3)

Western Blot: Podocalyxin Like Antibody (3D3) [NBP2-25219]

Western Blot: Podocalyxin Like Antibody (3D3) [NBP2-25219]

Western Blot: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3).
Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) [NBP2-25219]

Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) [NBP2-25219]

Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Confocal Immunofluorescence of HeLa cells using Podocalyxin Like Antibody (3D3) labeled with CF488 (Green); Red Dot is used to label the nuclei.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (3D3).
View 3 more images

Applications for Podocalyxin Like Antibody (3D3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Podocalyxin

Podocalyxin, also known as podocalyxin-like or PDXL, is a member of the CD34 transmembrane sialomucin family. Like other sialoglycoproteins, podocalyxin plays important roles in mediating cellular migration and cell-cell adhesion. Podocalyxin was first discovered in podocytes, the terminally differentiated epithelial cells lining the kidney glomeruli. Podocalyxin is expressed at high levels on the podocyte foot projections and plays essential roles in kidney development and homeostasis, particularly blood filtration and urine formation.
Podocalyxin is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons (reviewed in Nielsen, 2009). Aberrant expression of podocalyxin has been described in a variety of cancers including breast, prostate, liver, pancreatic, kidney and leukemia. Human podocalyxin migrates as a 165/170 kDa doublet on SDS-PAGE; however there are many post-translational modifications which may impact its actual size (Kershaw, 1997).
Antibody to podocalyxin has been reported to be a useful biomarker for various pathological conditions. For example, podocalyxin antibody can identify podocytes in the urine, also known as podocyturia, which can be indicative of glomerular disease, pre-eclampsia, and other kidney pathology (reviewed in Chen, 2012). Additionally, podocalyxin antibody studies have indicated that highly podocalyxin-positive tumors or podocalyxin dysregulation in general can be potentially linked to more aggressive tumors and poorer outcomes (Nielson, 2009). Hence, podocalyxin antibody may be used as part of a biomarker antibody panel to study expression of proteins associated with tumorigenesis. The podocalyxin antibody clones 3D3, 4F10, and 2A4 have been extensively published in the literature and researchers are encouraged to consult the published literature for additional information.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

UniProt

NP_001018121 (Human), O00592 (Human)

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (3D3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Podocalyxin Like Antibody (3D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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