Podocalyxin Like Antibody (3D3) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33108APC
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Rabbit
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3D3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27771514).
Localization
Cell surface and Cytoplasmic
Specificity
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Marker
Hematopoietic Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Scientific Data Images for Podocalyxin Like Antibody (3D3) [Allophycocyanin]
Flow Cytometry: Podocalyxin Antibody (3D3) [Allophycocyanin] [NBP2-33108APC] - An intracellular stain was performed on SH-SY5Y cells with Podocalyxin (3D3) antibody NBP2-33108APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Unstained cells are shown in black. Both antibodies were conjugated to allophycocyanin.
Applications for Podocalyxin Like Antibody (3D3) [Allophycocyanin]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Podocalyxin
Alternate Names
GCTM, PCLP, POD XL, PODXL
Gene Symbol
PODXL
Additional Podocalyxin Products
Product Documents for Podocalyxin Like Antibody (3D3) [Allophycocyanin]
Product Specific Notices for Podocalyxin Like Antibody (3D3) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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