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Podoplanin Antibody (8.1.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1015

Novus Biologicals, part of Bio-Techne
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NB600-1015
NB600-1015SS

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Human (Negative)

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Electron Microscopy, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Electron Microscopy, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Golden Syrian Hamster IgG Clone # 8.1.1

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Murine thymic stromal cell lines

Reactivity Notes

Does not cross-react with human.

Localization

Membrane; Single-pass type I membrane protein.

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Golden Syrian Hamster

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Podoplanin Antibody (8.1.1) - BSA Free

Western Blot: Podoplanin Antibody (8.1.1)BSA Free [NB600-1015]

Western Blot: Podoplanin Antibody (8.1.1)BSA Free [NB600-1015]

Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin is differentially expressed in primary podocytes relative to primary PECs after six passages in culture (arrow), consistent with mRNA expression analysis. Lysates of isolated glomeruli are used as positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22529955) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 5 ug/ml overnight at 4C and detected with an anti-Golden Syrian hamster DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin and these are cells of a malignant serosal tumor. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)

Applications for Podoplanin Antibody (8.1.1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:400

Immunocytochemistry/ Immunofluorescence

1-5ug/ml

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000-1:2000
Application Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NB600-1015 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Entrez Gene IDs

14726 (Mouse)

Gene Symbol

PDPN

UniProt

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (8.1.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Podoplanin Antibody (8.1.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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