Podoplanin Antibody (8.1.1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80916

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

CyTOF-ready, Electron Microscopy, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Golden Syrian Hamster IgG Clone # 8.1.1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Podoplanin Antibodies »

Product Specifications

Immunogen

Murine thymic stromal cell lines

Reactivity Notes

Does not cross-react with human.

Localization

Membrane; Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Golden Syrian Hamster

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Podoplanin Antibody (8.1.1) - Azide and BSA Free

Western Blot: Podoplanin Antibody (8.1.1)Azide and BSA Free [NBP2-80916]

Western Blot: Podoplanin Antibody (8.1.1)Azide and BSA Free [NBP2-80916]

Western Blot: Podoplanin Antibody (8.1.1) - Azide and BSA Free [NBP2-80916] - Analysis of Podoplanin in mouse kidney tissue extract. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - Azide and BSA Free [NBP2-80916]

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - Azide and BSA Free [NBP2-80916]

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) - Azide and BSA Free [NBP2-80916] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue). Image from the standard format of this antibody.
Immunohistochemistry: Podoplanin Antibody (8.1.1) - Azide and BSA Free [NBP2-80916]

Immunohistochemistry: Podoplanin Antibody (8.1.1) - Azide and BSA Free [NBP2-80916]

Immunohistochemistry: Podoplanin Antibody (8.1.1) - Azide and BSA Free [NBP2-80916] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito. Image from the standard format of this antibody.
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Applications for Podoplanin Antibody (8.1.1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:400

Immunocytochemistry/ Immunofluorescence

1-5ug/ml

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000-1:2000
Application Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (8.1.1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Podoplanin Antibody (8.1.1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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