POLR2E Antibody (OTI3B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00482

Novus Biologicals, part of Bio-Techne
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NBP2-00482
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-73518AF350
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Alexa Fluor 405
NBP2-73518AF405
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Alexa Fluor 488
NBP2-73518AF488
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Alexa Fluor 532
NBP2-73518AF532
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Alexa Fluor 594
NBP2-73518AF594
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Alexa Fluor 647
NBP2-73518AF647
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Alexa Fluor 700
NBP2-73518AF700
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Alexa Fluor 750
NBP2-73518AF750
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Allophycocyanin
NBP2-73518APC
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Allophycocyanin/Cy7
NBP2-73518APCCY7
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Biotin
NBP2-73518B
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CoraFluor 1
NBP2-73518CL1
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DyLight 350
NBP2-73518UV
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DyLight 405
NBP2-73518V
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DyLight 488
NBP2-73518G
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DyLight 550
NBP2-73518R
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DyLight 594
NBP2-73518DL594
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DyLight 650
NBP2-73518C
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DyLight 680
NBP2-73518FR
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DyLight 755
NBP2-73518IR
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FITC
NBP2-73518F
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HRP
NBP2-73518H
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Janelia Fluor 525
NBP2-73518JF525
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Janelia Fluor 549
NBP2-73518JF549
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Janelia Fluor 585
NBP2-73518JF585
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Janelia Fluor 635
NBP2-73518JF635
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Janelia Fluor 646
NBP2-73518JF646
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Janelia Fluor 669
NBP2-73518JF669
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mFluor Violet 450 SE
NBP2-73518MFV450
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mFluor Violet 500 SE
NBP2-73518MFV500
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mFluor Violet 610 SE
NBP2-73518MFV610
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PE
NBP2-73518PE
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PE/Atto594
NBP2-73518PEATT594
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PE/Cy5.5
NBP2-73518PECY55
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PE/Cy7
NBP2-73518PECY7
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PerCP
NBP2-73518PCP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B5

Concentration

0.9 mg/ml

View all POLR2E Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2E Antibody (OTI3B5)

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - POLR2A is destabilized by inhibition of oncogenic BRAFV600E signaling. Representative immunoblot of A375-P cells treated with PLX4720 or DMSO control for 16 hours, followed by cycoheximide treatment for the indicated timepoints. The lower panel shows the quantification of three independent biological replicates, normalized to the respective 0 CHX timepoint and alpha-Tubulin loading control (mean +/- SD). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-44112-7), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Human tonsil.
Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482]

Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482]

Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2E antibody, and then analyzed by flow cytometry.
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Applications for POLR2E Antibody (OTI3B5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR2E

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].

Alternate Names

DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1

Entrez Gene IDs

5434 (Human)

Gene Symbol

POLR2E

Additional POLR2E Products

Product Documents for POLR2E Antibody (OTI3B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR2E Antibody (OTI3B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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