POLR2E Antibody (OTI3B5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73518
![Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Western-Blot-NBP2-73518-img0001.jpg "Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]")
![Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Western-Blot-NBP2-73518-img0002.jpg "Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3B5
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for POLR2E Antibody (OTI3B5) - Azide and BSA Free
Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]
Western Blot: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0012.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody.![Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73518-img0004.jpg "Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-POLR2E antibody, and then analyzed by flow cytometry.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0005.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0006.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0007.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0008.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0009.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0010.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0011.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
![Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.bio-techne.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73518-img0003.jpg "Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Applications for POLR2E Antibody (OTI3B5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: POLR2E
Alternate Names
DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1
Gene Symbol
POLR2E
Additional POLR2E Products
Product Documents for POLR2E Antibody (OTI3B5) - Azide and BSA Free
Product Specific Notices for POLR2E Antibody (OTI3B5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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